Optimization of Radioimmunoassay for Human Growth Hormone by the Charcoal-Dextran Technique
نویسندگان
چکیده
منابع مشابه
[Radioimmunoassay for human growth hormone].
Detailedmethodsfor: (1) 1311 iodination of human growth hormone (HGH); (2) purification of labeled HGH (HGH-139); and (3) radioimmunoassay of HGH are given. Bio-GeI filtration is introduced as a rapid and reproducible method for purifying HGH-1311which hasbeenobtainedby a modificationof the methodof Greenwood at al. (1). Highlypurified HGH-’311 with a bindability of 96% or more is usually achie...
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A modification of the method for radioimmunoassayof insulin is described, which achievessatisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution fo...
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The use of activated charcoal in the radioimmunoassay of human growth hormone is described. The method permits the rapid separation of bound from free labelled human growth hormone in large batches and affords a quick method for screening iodination eluates. The necessity for an equality of protein concentration for the incubation and the separation procedure is emphasized. The method is simple...
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this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
[Radioimmunoassay of human growth hormone by adsorption to talc].
Radioimmunoassay of human growth hormone (HGH) using adsorption to talc was examined. Results are as follows: 1) The minimal detectable amount of HGH was 0.02 ng/ml. The mean recovery rate was 100.8%. Mean +standard error (S.E.) of the same two samples, repeatedly measured eight times, were 3.5+0.2 and 35.4+0.5 ng/ml, respectively . Concentrations of the same acromegalic plasma diluted in diffe...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1970
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/16.10.845